Abstract
The influence of adipate concentration and dissolved oxygen on production of adipoyl-7-aminodeacetoxycephalosporanic acid (ad-7-ADCA) by a recombinant strain of Penicillium chrysogenum expressing the expandase gene from Streptomyces clavuligerus was studied in glucose-limited continuous cultures. Operating conditions were maintained constant but the adipate and dissolved oxygen concentrations (DOC) were varied separately in a range from 1 to 37.5 g l −1 and from 2% to 125% air saturation (%AS), respectively. The total β-lactams specific productivity, r p total , was not significantly changed for adipate concentrations from 5 to 25 g l −1 , but the flux towards an unknown by-product decreased as the adipate concentration increased. Investigations at different DOC showed that r p total was stable around 18 μmol g DW −1 h −1 for DOC being in the range from 15 to 125%AS. When DOC was decreased from 15 to 7%AS, r p total increased to 25 μmol g DW −1 h −1 , mainly due to a two-fold increase in the adipoyl-6-aminopenicillanic acid (ad-6-APA) specific productivity.
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