Abstract
Rats are often used for experimental studies on fracture healing. Mostly, the rats were sacrificed after a certain time point and the fracture calli were investigated via biomechanical torsion testing, μCT and histology [Strube, 2008]. The easiest way to perform ex vivo torsion testing is to fix the long bone at the distal end in all six degrees-of-freedom (DOF), to fix the proximal end in all DOF except torsion, to apply a torsion moment to the proximal end and to measure the torsion angle. However, this might lead to an error in the measured torsional stiffness due to the parallel axis theorem if the specimen's axis of gravity is shifted or tilted with reference to the fixed axis of rotation during testing. As a result, significant differences in the fracture healing outcome between two animal groups might be undetected. The aim of this study was to quantify this error in the torsional stiffness via finite element (FE) voxel models of rat fracture calli after 35 days of healing.
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