Influence of temperature variation on the metabolism of pig muscle in situ and after excision

Abstract

The experiment involved six halothane-positive (HP) and six halothane-negative (HN) pigs of about 100 kg liveweight. Under general anaesthesia the tibialis cranialis was exposed. The temperature of the muscle was maintained at either 35 or 40°C ( in situ). The muscle was stimulated via the peroneal nerve at 0·1 Hz for 10 min then 1 Hz for 10 further min. Twitch contractions were recorded throughout the stimulation, after which the muscle was excised and split into two parts which were kept in paraffin oil at either 35 or 40°C (post-excision temperature) for 2 h. Samples were taken just before and after the 20 min stimulation period, and at 1 and 2 h after muscle excision for determination of pH and concentrations of PC, ATP, IMP, glycogen, G-6-P and lactate. As soon as one muscle was removed, the operation was repeated on the other leg. Both temperatures (35 and 40°C) were applied to each animal in a balanced design. However data were obtained from only 11 pigs at 40°C as one HP pig died accidentally at the beginning of the 40°C experiment. Halothane sensitivity influenced the pH value and the levels of PC, G-6-P and lactate of the muscle in situ ( P < 0·01 in all cases). Temperature affected contrction time ( P < 0·01). Both halothane sensitivity and Post-excision temperature affected the pH values and the levels of PC, ATP, IMP, G-6-P and lactate ( P < 0·01 in all cases) in the excised muscle. By contrast, the in situ temperature treatments did not affect any of the muscle traits measured after excision. It was concluded that the effects of the various treatments on the rate of metabolism in the excised muscle were wholly explainable in terms of temperature from the time of excision, and that the in situ temperature treatments may not be responsible for the differences after excision.