Abstract
The effect of temperature on the separation of trace amounts of substances by ion exchange chromatography is discussed. The overall influence of temperature on the final resolution of chromatographic peaks consists of corresponding contributions due to the changes of separation factor and of plate height. Both separation factor and plate height can either decrease or increase or remain constant with a rise in temperature depending on the species to be separated and the ion exchange system employed. A general classification of possible changes in resolution resulting from the variation of separation factor and plate height has been given, and numerous examples are presented.
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