Abstract
The aim of this research was to study the effect of temperature and carbon sources for synthesizing activity of Rhodococcus erythropolis VKM Ac-2017D strain on the mineral medium. Tryptophan at a concentration of 0,5 g/l was added as a precursor for indole-3-acetic acid (IAA) biosynthesis. Phytostimulating effect was shown in condition of soil experiment using lawn grass mixture (Festuca rubra 30%, Lolium perenne 30%, Poa pratensis 20%, Festuca rubra trichlorophylla 15%, Festuca ovina 5%). The strain of selected oil-degrading bacteria was able to produce IAA on both hydrocarbon and carbohydrates as carbon sources. The differences were found in the production of auxin that depended on temperature and types of C-substrate. When sucrose used as a carbon source the maximum in production of IAA was 34,3 g/ml at the temperature of 15C. When culturing microorganisms on hexadecane the highest concentration IAA (77,69 g/ml ) was achieved at 25C. The culture broth (supernatant) and native bacterial suspension had shown phytostimulating activity on the lawn grass (weight of plant biomass, germination) to compare to water and chemically synthesized IAA in condition of soil experiment.
Highlights
relevant to reveal some aspects of its behavior
The important point is the adaptation of the species in the conditions
which are observed in the national park's territory
Summary
При культивировании микроорганизмов на гексадекане максимальный выход фитогормона отмечен при температуре 25°С, и он составил 77,69 мкг/мл. Оценку фитостимулирующих свойств штамма бактерии Rhodococcus erythropolis ВКМ Ас-2017D проводили в полевом эксперименте – в почвенных условиях урбанозёма с высевом газонной травосмеси. Участок No 1 – культуральная жидкость, отобранная как супернатант после центрифугирования исходной жидкой культуры; No 2 – биомасса клеток, осажденная ценрифугированием; No 3 – нативная жидкая культура (ЖК) исследуемого штамма; No 4 – раствор химически синтезированной ИУК, равный по концентрации содержанию ИУК в бесклеточной жидкости (50 мг/л); No 5 – контроль (соответствующий объем воды) Rhodococcus erythropolis ВКМ Ас-2017D способен к продукции фитогормона ауксина как на углеводородном, так и на углеводном источнике углерода. В полевом эксперименте показано, что максимальной фитостимулирующей активностью по отношению к газонной траве в условиях урбанозёма обладали супернатант и нативная жидкая культура штамма Rhodococcus erythropolis ВКМ Ас-2017D.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
