Abstract

The efficacy of two selected fungicides i.e., tebuconazole and coppoer hydroxide, was conducted experiments in laboratory and copper hydroxide on the two specific enzymes phosphatase and urease were determined in two different soil samples (red sandy loam and black clay soils) of groundnut (Arachis hypogaea L.) from cultivated fields of Anantapuramu District, Andhra Pradesh. The activities of the selected soil enzymes were determined by incubating the selected fungicides-treated (1.0, 2.5, 5.0, 7.5 and 10.0 kg ha−1) and -untreated groundnut soil samples at 10 day intervals. By determining the effective concentration, the rate of selected enzyme activity was estimated by adding the suitable substrate at 10, 20, 30 and 40 days of soil incubation. Both the enzyme activities were increased up to 5.0 kg ha−1 level of fungicide in both soil samples significantly at 10 days of soil incubation and further enhanced up to 20 days of incubation. The activity of the phosphatase and urease decreased progressively at 30 and 40 days of incubation. From overall studies, higher concentrations (7.5 and 10.0 kg ha−1) of both tebuconazole and copper hydroxide were toxic to phosphatase and urease activities, respectively, in both soil samples.

Highlights

  • In India, it has become a common trend in modern agriculture to apply agrochemicals such as pesticides to increase agricultural productivity and has become an integral part of agriculture

  • The efficacy of two selected fungicides i.e., tebuconazole and coppoer hydroxide, was conducted experiments in laboratory and copper hydroxide on the two specific enzymes phosphatase and urease were determined in two different soil samples of groundnut (Arachis hypogaea L.) from cultivated fields of Anantapuramu District, Andhra Pradesh

  • Our investigation revealed that phosphatase and urease activities were drastically decreased at higher concentrations (7.5 and 10.0 kg ha-1) of tebuconazole and copper hydroxide-treated soils than the untreated controls throughout the experiment

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Summary

Introduction

In India, it has become a common trend in modern agriculture to apply agrochemicals such as pesticides to increase agricultural productivity and has become an integral part of agriculture. Different groups of pesticides with various formulations have been introduced into agricultural soils either simultaneously or in succession to protect crops from weeds, insects, and fungi. Pesticides have beneficial impacts in improving and stabilizing agricultural productivity by controlling pests; these organic chemicals may contaminate the soil ecosystem and various groups of organisms in soil, which play a vital role in mineralization, nitrification and phosphorus recycling. Among the different groups of organisms, microorganisms play an important role in many soil biological processes; soil enzymes play various biochemical functions in the overall process of organic matter decomposition in the soil system (Burns 1983; Sinsabaugh et al 1991). The soil activities undergo complex biochemical processes that consist of integrated and ecologically connected synthetic processes, immobilization and enzyme stability (Khaziyev and Gulke 1991).

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