Influence of sucrosis on the degradation of pesticide by white rot fungus

Victória Maura Silva Bermúdez,Kelly De Araujo Rodrigues Pessoa,Lia Teles Lima,Barbara Chaves Aguiar Barbosa,Leticia Bezerra Farias,Gloria Maria Marinho Silva

doi:10.33448/rsd-v10i15.22790

Victória Maura Silva Bermúdez, Kelly De Araujo Rodrigues Pessoa + Show 4 more

Open Access

https://doi.org/10.33448/rsd-v10i15.22790

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Abstract

The influence of sucrose on the removal of Paraquat (PQT) in synthetic aqueous medium was evaluated by Phanerochaete chrysosporium. Initially, a toxicity test was performed on plates containing paraquat at concentrations of 1, 5, 10, 20 and 30 mg.L-1. Then, they were carried out in batches - agitated batch (RBA) and sequential batch (RBS). Four reactors were submitted, containing medium with 30 mg.L-1 of paraquat, under a reaction time of 144 h, the reactors being RBA-2 and RBS-2 with the addition of 2 gL-1 of sucrose, and without the adding sucrose to the RBA-0 and RBS-0 reactors. In all reactors, paraquat was removed, but in RBS-0, the best mean removal efficiency was obtained (41.1 ± 0.89%). The best values of apparent speed of degradation (k) were found in reactors with sucrose RBA-2 and RBS-2, 0.015 ± 0.002 h-1 and 0.018 ± 0.002 h-1, respectively, indicating that the addition of sucrose influenced the speed removal of paraquat. It was also verified that the pollutant was not completely removed by adsorption to fungal biomass, which microorganisms predominated in the medium at the end of the treatment, demonstrating their role in the paraquat bioremediation process. Therefore, the addition of sucrose influenced the removal speed of the PQT and COD, but not the removal efficiency.

Highlights

Environmental pollution from pesticide use is seen as a serious problem due to its adverse effect on human health, plants and animals (Galic et al, 2018; Pandiselvam et al, 2020)
3.1 Plaque Toxicity Test The toxicity step in plates aimed to evaluate the growth of fungal colonies for 7 days, with different concentrations of pesticide (Figure 1) and define the concentration to be used in sequential batch reactors
The test showed that colonies of the fungus Phanerochaete chrysosporium grew at all pesticide concentrations with maximum diameters of 8.4 cm (Figure 1)

Summary

Introduction

Environmental pollution from pesticide use is seen as a serious problem due to its adverse effect on human health, plants and animals (Galic et al, 2018; Pandiselvam et al, 2020). In regions of agricultural development, with high plantations, paraquat is the main source of pollution of water and soil resources (Sorolla et al, 2012) Exposure to this pollutant can cause serious side effects (Dong et al, 2013; Fukushima et al, 2002; Song et al, 2020) and has been used as a method of suicide (Wu et al, 2014). This active ingredient is banned in Brazil since September 22, 2020, as a result of a toxicological re-evaluation, as provided by Resolution RDC No 177 of September 21, 2017, published in the Official Gazette of the Union of September 22, 2017

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