Abstract
The kinetics of mutarotation of lactose in the presence of sucrose was determined at 25, 30 and 35C. The rate of mutarotation of lactose decreased slightly by sucrose added to 40% and thereafter greatly decreased. Increasing sucrose from 30 to 60g per 100ml solution, decreased the velocity constant 1.8 to 1.9 times, independent of the concentration of lactose. In a whey-salt solution, mutarotation was increased 1.8 to 1.9 times. With sucrose the whey-salt solution did not increase the rate constant and mutarotation rates were then essentially the same as in distilled water. Increasing sucrose decreased mutarotation constants in the whey-salt solution as in distilled water. Effect of sucrose was apparent at 25g per 100ml solution. Potassium dihydrogen phosphate, Na3-citrate, and K3-citrate catalyzed the mutarotation velocity of lactose, did not increase the rate constant with 30g sucrose per 100ml solution.
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