Abstract
Increasing concentrations of sucrose stimulate β-GS activity in heavy and light membranes of roots of Lupinus. Measured β-GS activity in fractions obtained by sucrose gradient centrifugation should hence be corrected when used as a test for plasmalemma identification. After applying corrected β-GS values, fractions enriched in plasmalemma can be detected in both heavy and light membrane preparations at lower densities of sucrose than those usually admitted. The sucrose effect seems largely due to an osmotic shock and so it would be preferable to use non-osmotic media for membrane purification on gradients.
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