Abstract
An influence of different sucrose concentrations in the culture media on the photosynthetic parameters, photosynthetic apparatus related genes expression, oxidative processes and acclimation of grape plants cultured in vitro was examined in this article. An increase of the sucrose concentration in the culture media resulted in a reduced expression of several photosynthetic genes. The most effective functioning of the photosynthetic apparatus was discovered by a decreased amount of surcose in culture media. An increase of the sucrose concentration in the culture media disrupts pigments synthesis, particularly carotenoids, which can be a cause of the secondary oxidative stress formation and grape plants growth reduction during acclimation.
Highlights
Clonal micropropagation is a promising method for obtaining healthy planting material, replicating the required number of plants in a short time
In vitro plants are grown in certain conditions: high relative humidity, low light, constant temperature, increased sugar concentrations in the culture media, growth regulators and nutrients, which countribute to the plant growth [1]
An increased content of the photosynthetic pigments alongside with the elevated sucrose concentration in the culture media was established in 28 DC plantlets, which can be linked to the increased availability of organic compounds
Summary
Clonal micropropagation is a promising method for obtaining healthy planting material, replicating the required number of plants in a short time. In vitro plants are grown in certain conditions: high relative humidity, low light, constant temperature, increased sugar concentrations in the culture media, growth regulators and nutrients, which countribute to the plant growth [1]. Plantlets lose their transpiration regulating mechanisms while adapting to the in vitro environment single [2], resulting in an abnormal anatomy and leaf morphology, poor granas development [3] and low photosynthesis rates [4]. Sucrose usually serves as a carbon source during clonal micropropagation and ensures in vitro plants growth and development. It induces an oxidative stress, which can increase plant death rate [7, 8]
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