Abstract
ObjectivesTo investigate the influence of storage methods of whole blood samples on Deoxyribonucleic acid (DNA) quality. Method and materialsWhole blood samples were collected from 90 patients reported to the Government Tertiary-care Dental College and Hospital, Tamil Nadu, India. The blood samples were stored at three different temperatures −80°C, −20°C, 4°C which were analyzed for DNA quality immediately within 4h and after a storage period of 60 days. Quantification of DNA in the sample was recorded using a spectrophotometer. ResultsThe average concentration of isolated DNA ranged from 0.5ng/μl to 154.2ng/μl with purity A260/A280 ratio ranging from 0.7 to 2.0. The mean DNA concentration H=97.2 (p=0.001) and purity H=80.1 (p=0.001) obtained from whole blood stored at 4°C were statistically significant. Thus samples stored at 4°C did not affect on DNA yield and quality. ConclusionWe conclude that storage at 4°C temperature for whole blood does not substantially affect the molecular assay measurements. This study enlightens the influence of storage temperatures and duration in genetic epidemiology.
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