Influence of spawning on swimming performance and muscle contractile properties in the short‐horn sculpin

Abstract

The total distance travelled during the first two kinematic stages of the escape response of short‐horn sculpin was significantly greater in post spawning fish (0·41 L) than in gravid fish (0·23 L). The maximum velocity of the snout during the C‐bend was significantly higher (5·6 L s−1) in postspawning fish than in gravid fish (3·8 L s−1). To investigate some of the mechanisms underlying changes in swimming performance, the contractile properties of fast muscle fibres were determined in fish of similar body length. The rate of tetanic force relaxation (time from last stimulus to 50% peak force) was 34% faster in gr avid than in postspawning fish. Maximum contraction velocity, determined by the slack‐test method, was significantly higher in gravid than in postspawning fish (6·8 v. 5·9 muscle lengths s−1). In contrast, both maximum isometric stress and power output (determined from the force–velocity relationship) were >50% higher in fibres from postspawning than from gravid fish, even though myofibrillar protein and water contents were similar (120 mg g−1 wet mass and 86% of body mass, respectively). The results show that swimming performance and the contractile properties of fast muscle fibres vary with the reproductive cycle in short‐horn sculpin acclimated to the same photoperiodic and temperature regime.