Influence of sorghum residues and tillage on soil organic matter and soil microbial biomass in an australian vertisol

Abstract

Short- and medium-term changes in soil organic matter content following a change in soil management or land use are often difficult to measure because they occur slowly against a large background of soil organic matter which can have considerable spatial variability. Results from an experiment with grain sorghum ( Sorghum bicolor L.) on a vertisol in sub-tropical Australia demonstrate the usefulness of two techniques for detecting trends in surface soil organic matter before they can be assessed by conventional methods. Firstly, using soil microbial biomass C as a sensitive indicator of changes in soil organic matter. Secondly, by using initial values of soil organic C or total N, measured before imposition of treatments, as a covariate in an analysis of variance. The combination of these techniques provided the most sensitive approach for detecting changes. The above-ground residues of sorghum (41 dry matter ha −1) were either retained or removed from plots that received conventional or zero tillage for 6 yr. Averaged over tillage treatments, soil organic C in the surface 0–10 cm layer was 8% greater in the residue-retained than in the residue-removed treatment, a difference equivalent to 16% of the C added as residues. The trend to increased soil total N was not significant. Residue retention caused larger percentage increases in microbial biomass C, measured by the chloroform fumigation-incubation method, than in total organic C and total N. The increase in biomass C was 12%, biomass N 23% and biomass P 45%, equivalent to 0.7% of the C, 7% of the N and 32% of the P added in residues. Residue retention decreased the biomass C-to-P ratio from 48 to 35, but these values were still much wider than those previously measured in U.K. soils. Residue retention increased respiration by about 45% (measured by CO 2 evolution during a 30-day incubation) but had little effect on biomass C-to-N ratio or mineralization of N. Averaged over the two residue management treatments, soil organic C in the surface 10 cm layer was 7% greater under zero tillage than under conventional tillage. The corresponding increase in biomass C was 14–21%, but there were no differences in biomass N or biomass P. CO 2 evolution and specific respiration by the biomass (μ g CO 2-C evolved g −1 biomass C day −1) were less in zero-tilled than in conventionally tilled soils. The combined effects of residue retention and zero tillage caused increases of 15% in surface soil organic C, 18% in soil total N and 31% in biomass C.