Abstract
In natural conditions, insertion of Agrobacterium T-DNA into the plant genome and its subsequent transfer via sexual reproduction has been shown for several dozens of species, including species from genera Nicotiana and Vaccinium. In the framework of investigation of possible function of cT-DNA in naturally transgenic species we have shown, that increasing of expression of rolC in Nicotiana tabacum is associated with increase of amount of glucose and total sugar content. Similar trend was observed for rolB/C-like gene in Vaccinium.
Highlights
Agrobacterium-mediated transformation is the key method of genetic engineering of plants aimed to introduce novel genes into their genomes
It is worth noting that all previously studied by us samples of V. macrocarpon contain intact gene while all samples of V. oxycoccos contain a deletion within the plast gene
Transgenic N. tabacum plants, containing rol C under dexamethasone-inducible promoter and control non-transgenic plants were cultivated for 7 days on MS media supplied with 10 μM of dexamethasone or on MS0 media [9]
Summary
Agrobacterium-mediated transformation is the key method of genetic engineering of plants aimed to introduce novel genes into their genomes. At the same time in natural conditions, horizontal transfer of T-DNA genes into the plant genomes has been shown for several dozens of species, including species from genera Nicotiana and Vaccinium [1,2,3,4]. They are mostly described in T-DNAs. The plast genes can modify plant growth in different ways, but the molecular basis of their function remains largely unknown. Just a few agrobacterial plast genes, including rolB, rolC, orf13 and 6b, have been studied in detail.
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