Abstract
ABSTRACT Four experiments were performed to determine the effects of external Ca++, L-cysteine, ammonia, and contact with glass surfaces on the acrosome reaction of spermatozoa of Homarus americanus. Few of the spermatozoa incubated in Ca++-free sea water or in sea water containing L-cysteine showed an acrosome reaction. Many acrosome reactions were observed among spermatozoa incubated in sea water containing Ca++ or ammonia and also when Ca++ was added to spermatozoa suspended in Ca++-free sea water. It is concluded that Ca++ plays an important role in this lobster's acrosome reaction. Incidences of acrosome reactions were higher in suspensions of spermatozoa in Ca+ +-free sea water that were agitated than in those that were not agitated.
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