Influence of silver carp skins processing conditions on collagen structure

Abstract

Infrared (IR) spectroscopy is currently one of the main methods for the analysis and identification of organic compounds. The method is based on passing infrared radiation through the object under study, which entails the excitation of molecules, forcing them to make oscillatory movements. During this process, a decrease in the intensity of light passing through the sample is observed. Absorption occurs at long waves, the energy of which is comparable with the excitation energy of vibrations in the molecules under study. This indicates the presence in the molecules of samples or functional groups. Thus, you can predict and find out about the possible structure of the substance. The authors of the study proposed a method for producing a collagen-containing base. The selection of the concentration of organic acid makes it possible to obtain a product with the necessary characteristics. The main objective of the experiment is to characterize the structural changes in the collagen fiber in the process of obtaining collagen hydrate. Infrared spectra were obtained at each stage, starting from the initial sample and ending with the final collagen hydrate. A comparative characteristic of the collagen molecules of the initial and final samples allows us to conclude that the aggressive medium in the process of hydrate production does not in the least damage the native structure of collagen fibrils. In the process of obtaining collagen fibers, they are released. Subsequently, the collagen bases were homogenized in a medium with distilled water, using the ratio: one part of the mass of processed silver carp skins and three parts of water. Obtaining an emulsion with high hydrophilicity, which creates the conditions for use in various forms in the food, cosmetic and medical industries.