Influence of short‐term nutritional overload on inflammationrelated gene expression profile in adipose tissue of Wistar rats

Abstract

High consumption of fat and sugar is linked to obesity and induces adipocyte to secrete proinflammatory cytokines. However, it is unclear when this process occurs. The aim was to determine the effect of short‐term of high‐saturated fat diet plus sugar on obesity and inflammatory cytokines gene expression in adipose tissue. Male Wistar rats (body weigth =348±34g) received standard chow (C, 25% protein, 58% carbohydrate and 5% fat 17%, with 17% saturated and 82% unsaturated) or high‐fat diet (H, 21% protein, 45% carbohydrate, 29% fat with 50% fat saturated and 50% unsaturated fat) plus sugar in the water (30%). Obesity was characterized by adiposity index (%). Was assessed gene expression of inflammation‐related gene (IL‐6, TNF‐α and MCP‐ 1). H rats showed an adiposity index higher than C (C= 4,6 ±0,9 vs H= 7,7±1,5%, p< 0,001). There was no diference between groups for inflammation‐related gene in adipose tissue. The short‐term of nutritional overload leads to obesity, but was not enough to alter the inflammation‐related gene expression profile in epididymal adipose tissue.