Influence Of Short, Disrupted Sleep And High-intensity Interval Exercise On Fasting And Post-prandial Blood Lipid And Lipid-related Antioxidant Responses In Healthy Men

Abstract

Exercise is known to impart transient blood lipid responses that appear consistent with reduced cardiovascular disease risk; yet, it is unclear how short, disrupted sleep (SDS) modifies post-exercise fasting and postprandial blood lipid and lipid-related antioxidant responses to a single episode of exercise. PURPOSE: To determine the influence of a single night of SDS on fasting and postprandial blood lipid and lipid-related antioxidant responses after HIIE. METHODS: Fifteen male participants (age 31.1 ± 5.3 yr; weight 83.5 ± 11.4 kg; BMI 25.8 ± 2.7 kg/m2; VO max 49.1 ± 8.5 ml/kg/min) completed a non-exercise control trial after 9 to 9.5 hrs of reference sleep (REF), HIIE by treadmill running (90% and 40% of VO2reserve in 3:2 min ratio) to expend 500 kcals after reference sleep (REF+EX) and HIIE after 3 to 3.5 hrs of short and disrupted sleep (SDS+EX) in a randomized crossover design. Blood samples were obtained by the same technician under standardized conditions just before, immediately after (IPE), 1 hr after exercise (1 HR) and just before a high-fat meal - 1240 kcals (56 g fat; 145 g carbohydrate; 38 g protein) and again 2, 4 and 6hrs after meal ingestion and at equal intervals during REF. Total, high-density and low-density lipoprotein cholesterol (HDLC and LDLC) and paraoxonase-1 concentration were measured up to 1 hr post-exercise. Post-prandial triglyceride was measured and area under the curves - total (AUCt) and incremental (AUCi) were calculated. Lipid and lipid-related antioxidant responses were analyzed using 3 (condition) by 3 (sample point) repeated measures ANOVAs. AUCt and AUCi were measured using one-way, 3 (condition) repeated measures ANOVAs. RESULTS: HDLC (+6.3%, p = 0.0023) and paraoxonase-1 (+10.8%, p <0.0001) increased and triglyceride (-18.5%, p <0.0001) decreased after REF+EX and SDS+EX; TAUCt and AUCi remained refractory to exercise and short, disrupted sleep. SUMMARY: Exercise transiently increased fasting HDL cholesterol and related antioxidant concentrations and reduced triglyceride levels, but did not modify total or incremental triglyceride AUC in response to a post-exercise high-fat meal. Short, disrupted sleep did not influence these responses.