Abstract
Till now sexual features of cystathionine-γ-lyase/H 2 S system functioning in heart and its relationship to DNA content in myocardial cells nuclei depending on the level of sex hormones. The aim of this work was to evaluate the effect of sex hormones different levels on DNA fragmentation, indicators of cell cycle, cystathionine-γ-lyase expression and H 2 S content in heart in male and female rats. Materials and м ethods. The experiments were performed on 40 white laboratory rats of both sexes. Deficiency of sex hormones in the organism of rats was created by castration (ovariectomy and testectomy respectively to female and male rats) with surgical method. Estradiol and testosterone in blood plasma of the animals were determined by immunoenzyme method. DNA level in myocardial cells nuclei was determined by flow DNA cytometry. Cystathionine-γ-lyase gene expression was determined by real time polymerase chain reaction in (Real-time PCR). H 2 S content in heart was evaluated by spectrophotometry. Statistical processing of the obtained results was performed using standard methods by applying the software package MS Excel and Statistica SPSS 10.0 for Windows. Results . It has been found that in male rats expression of cystathionine-γ-lyase and H 2 S content in myocardium were significantly lower, by 46.9 and 16.1 % (p < 0.05) respectively, than in female rats. Along with this, males had greater activity of apoptosis, proliferation and polyploidization compared to females. Gonadectomy in males reduced expression of cystathionine-γ-lyase (47.0 %, p < 0.05), content of H 2 S in myocardium (by 22.2 %, p<0.05), activity of apoptosis, proliferation and polyploidization in heart, whereas castration of females caused opposite changes in relation to the control group. Gonadectomy in the animals changed the vector of sex differences in the studied parameters: cystathionine-γ-lyase expression and H 2 S content in myocardium of castrated males was significantly higher, whereas activity of apoptosis, proliferation, and polyploidization were less than in the relevant group of females. Conclusions. System of cystathionine-γ-lyase/H 2 S in myocardium is an important molecular target through which multidirectional sex hormones influence on DNA content in the nuclei of myocardial cells of rats is implemented.
Highlights
The aim of this work was to evaluate the effect of sex hormones different levels on DNA fragmentation, indicators of cell cycle, cystathionine-γ-lyase expression and H2S content in heart in male and female rats
Males had greater activity of apoptosis, proliferation and polyploidization compared to females
Gonadectomy in the animals changed the vector of sex differences in the studied parameters: cystathionine-γ-lyase expression and H2S content in myocardium of castrated males was significantly higher, whereas activity of apoptosis, proliferation, and polyploidization were less than in the relevant group of females
Summary
Що в самців щурів експресія цистатіонін-γ-ліази та вміст H2S у міокарді вірогідно менші відповідно на 46,9 та 16,1 % (р < 0,05), ніж у самок. Гонадектомія самців зменшує експресію цистатіонін-γ-ліази (на 47,0 %, р < 0,05), вміст H2S у міокарді (на 22,2 %, р < 0,05), активність апоптозу, проліферації та поліплоїдизації в серці, тоді як кастрація самок викликала протилежні зміни щодо контролю. Гонадектомія тварин змінює вектор статевих відмінностей досліджуваних показників: у кастрованих самців експресія цистатіонін-γ-ліази, вміст H2S у міокарді вірогідно більші, тоді як активність апоптозу, проліферації та поліплоїдизації менші, ніж у відповідної групи самок. Система цистатіонін-γ-ліаза/H2S у міокарді – важлива молекулярна мішень, через яку реалізується різно спрямований вплив статевих гормонів на вміст ДНК в ядрах клітин міокарда щурів. Остаются неопределенными половые особенности функционирования системы цистатионин-γ-лиаза/гидроген сульфид в сердце и ее связь с содержанием ДНК в ядрах клеток миокарда в зависимости от уровня половых гормонов. Цель работы – оценить влияние различного уровня половых гормонов на фрагментацию ДНК, показатели клеточного цикла, экспрессию цистатионин-γ-лиазы и содержание гидроген сульфида (H2S) в сердце у самцов и самок крыс
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