Abstract
The aim of this study was to analyze the influence of municipal sewage sludge from the Municipal Wastewater Treatment Plant in Sokolka on the content of magnesium and selected micronutrients such as zinc, manganese, copper and iron in the soil and lawn grasses and on the content of chlorophyll a and b in the analyzed plants. These elements are especially important in the process of photosynthesis. In addition, the paper discusses the relationship between the studied elements and the content of assimilation pigments. The experimental plots were sown with two lawn grass mixtures: Eko and Roadside, and three doses of sewage sludge 0.0 (control), 7.5 kg m -2 and 15.0 kg m -2 were used. Chlorophyll a and b content was determined using spectrophotometer by measuring absorbance at = 663 and 645 nm. Metals concentrations in soils with sewage sludge and in plant material were determined using Atomic Absorption Spectrometry. It was found that the sludge dose had a significant effect on the manganese content in the soil taken from the experiment in Piastowska Street. In the experiment in Popieluszki Street the application of sewage sludge had a significant impact on the content of zinc and copper in the studied mixtures of grasses and in Raginisa Street - on the contents of zinc, copper and manganese. The analysis of correlations for the results obtained from experiment in Hetmanska Street revealed strong positive correlations between the content of Zn and Mn in the soil and the content of chlorophyll a in plants (r = 0.84 and r = 0.83, respectively) and strong negative correlations between the content of Zn in the soil and the content of chlorophyll b in lawn grasses collected in June and August (r = -0.81 and r = -0.85, respectively). Strong correlations between the concentration of zinc and the content of chlorophyll b in grass samples collected in Hetmanska Street, Piastowska Street and Popieluszki Street (r = 0.85, r = 0.86 and r = 0.98, respectively) and the Cu content in plants and chlorophyll b for plants collected in Hetmanska Street (r = 0.97), Piastowska Street (r = 0.86), Popieluszki Street (r = 0.90) and Raginisa Street (r = 0.82). The content of chlorophyll b was differentiated by dose of sludge and sampling time. The highest average chlorophyll b content in the grass samples collected in June and July was found (1.10 mg g -1 of f.m.), and the lowest in samples collected in October (0.39 mg g -1 of f.m.). The content of chlorophyll a in the analyzed
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