Abstract
Cationic lipid-based nanoparticulate systems are delivery systems that has been widely used in pharmaceutical field including gene delivery. There are many barriers obstructing genetic materials and their delivery systems to reach the target. Serum is one of the imperative factor that should be investigated. Therefore, the aim of this study was to examine the effect of serum on DNA protection ability of spermine-liposomes and niosomes by evaluating the percentage of transfection efficiency in Hela cell and observing the DNA degradation band using agarose gel electrophoresis in the presence of serum. The results showed that the percentage of transfection efficiency of spermine-liposomes was dramatically decreased when serum is presented (p< 0.05). In contrast, whether or not the serum is presented, the spermine-niosomes showed no significant difference in transfection efficiency. Concisely, liposomes could slightly protect DNA from DNase in the serum, whereas, niosomes had potential ability to protect DNA from the enzymes in serum. This result revealed an advantage of the cationic niosomes system as a gene carrier over the cationic liposomes.
Highlights
Numerous researches have been using cationic lipid-based nanoparticles such as cationic liposomes and cationic niosomes for gene delivery which can be applied in various applications such as infectious diseases, metabolic diseases, cancers and regenerative medicines [1,3]
When the cationic lipids were prepared as niosomes, no significant effect of serum on the percentage of transfection efficiency has been observed (p > 0.05)
The DNA protection ability was investigated by gel agarose electrophoresis after complexing with cationic lipid-based nanoparticles (cLNPs)
Summary
Numerous researches have been using cationic lipid-based nanoparticles (cLNPs) such as cationic liposomes and cationic niosomes for gene delivery which can be applied in various applications such as infectious diseases, metabolic diseases, cancers and regenerative medicines [1,3]. Spermine-liposomes and niosomes exhibited a potential gene delivery and high transfection efficiency [4, 5]. Hypothesis of serum incompatibility with cLNPs begin through interaction between negative charge of serum proteins onto the positive charge of cationic systems affected electrostatic interaction with proteins on the cell surface and their internalization [8,9]. The objective of this research is to explore the effect of serum on the DNA protection ability of liposomes and niosomes by evaluating the percentage of transfection efficiency in Hela cell and observing the DNA protection ability by agarose gel electrophoresis in the presence of serum
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