Abstract
The plastic surfaces of tissue culture dishes bind about 5 × l0 2 molecules of concanavalin A (ConA)/μ 2. This binding is virtually uninhibited by alpha-methyl-mannoside (α-MM). If the plastic is treated with fetal calf or dog serum, binding of ConA increases to about 8 × 10 3 molecules/μ 2, but is almost completely inhibited by α-MM. The level of binding of ConA to human red blood cells (RBC)—about 1.4 × 10 4 molecules/μ 2—does not increase if cells are first treated with fetal calf or dog serum; human RBC treated with dog serum, however, are about 100 times more agglutinable by ConA than are untreated cells. Our results show that: ( a) serum components can increase the binding of ConA to inert surfaces; ( b) serum components can increase the sensitivity of human RBC to agglutination by ConA; and ( c) ConA can bind to surfaces by at least two mechanisms (one involving sites sensitive to inhibition by α-MM, and the other not).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
