Influence of selenium on root morphology and photosynthetic characteristics of winter wheat under cadmium stress

Abstract

To explore the feasibility of reducing cadmium (Cd) concentrations and alleviating Cd toxicity via selenium (Se), this study investigated the effects of Se on the root morphology, photosynthetic parameters, and Se, and Cd concentrations in winter wheat under different Cd stress levels. Three Cd levels (0, 5 and 10 μM) and three Se levels (0, 1 and 5 μM) were applied in a hydroponics test. The results showed that increases in Cd stress significantly decreased the shoot and root dry matter weight, root length, root volume, root effective surface area, root total surface area, Se concentration, Se accumulation, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr) and intercellular CO2 concentration (Ci). Increases in Cd stress significantly increased the Cd concentration and accumulation in shoots and roots, the root average diameter, and the stomatal limiting value (Ls). There were significant relationships between the root Cd concentrations and the root morphology parameters, shoot Cd concentrations and photosynthetic characteristics of winter wheat. The Se1 and Se5 treatments effectively increased the shoot dry matter weight, root length, root volume, root effective surface area and root total surface area and decreased the shoot Cd concentration, when Cd stress was at the medium level (Cd5). Only the Se1 treatment effectively increased the Gs, Tr and Ci under the Cd5 treatment. When Cd stress was at the high level (Cd10), the Se5 treatment had greater effects on root growth than the Se1 treatment, the Se levels had no pronounced effect on the shoot dry weight and Cd concentration in winter wheat. Meanwhile, the root Se concentration was closely related to each root morphology parameter. Cd stress inhibited the root growth and leaf photosynthesis of winter wheat, and a high enough Se supply reduced the Cd toxicity by enhancing the root growth of winter wheat.