Influence of Selected Factors on Biofilm Formation by Salmonella enterica Strains.

Agata Białucha,Joanna Kwiecińska-Piróg,Eugenia Gospodarek-Komkowska,Krzysztof Skowron

doi:10.3390/microorganisms9010043

Agata Białucha, Joanna Kwiecińska-Piróg + Show 2 more

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https://doi.org/10.3390/microorganisms9010043

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Abstract

Biofilm formed by S. enterica on the surface of gallstones or biomaterials promotes the development and spread of chronic infection. The aim of the study was to assess biofilm formation on the surface of polystyrene depending on nutritional conditions and the effect of 0.5, 1.0, and 2.0% glucose and 3.0% bile and sub-inhibitory concentrations of ampicillin on biofilm formation of S. enterica. Sixty-nine clinical strains of S. enterica isolated from feces (92.8%) and blood (7.2%) collected from patients (66.7%) and carriers (33.3%) were used in the study. Assessment of forming 24-h biofilm by these strains was performed on the surface of polystyrene 96-well plates at 37 °C. In this study, it was indicated that 1.0% glucose and 3.0% bovine bile inhibit biofilm formation. Biofilm formation was inhibited in all examined sub-MIC of ampicillin. Biofilm formation is varied in different conditions, depending on the serovar.

Highlights

Salmonella enterica is the prevailing bacterial etiological factor of food poisonings and gastrointestinal tract infections in the world
The aim of this study was the formation of 24-h biofilm on the surface of polystyrene in S. enterica strains, and the effect of glucose, bile salts, and sub-Minimal Inhibitory Concentration (MIC) concentrations of ampicillin on this process
Based on the criteria of biofilm formation intensity described in the previous section, the highest percentage of strains formed weak biofilm (36, 52.2%)

Summary

Introduction

Salmonella enterica is the prevailing bacterial etiological factor of food poisonings and gastrointestinal tract infections in the world. Main sources of these bacteria include products of animal origin and animal feces which must be subjected to hygienization in order to provide sanitary and hygienic safety [1,2]. Both patients and carriers play an important role in the transmission of these pathogens. Treatment of infections with the participation of biofilm formed by Salmonella spp. has limited effectiveness [7,8]. It is necessary to seek effective methods for its prevention and eradication

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