Abstract

Carbon nanostructures have recently gained significant interest from scientists due to their unique physicochemical properties and low toxicity. They can accumulate in the liver, which is the main expression site of cytochrome P450 (CYP450) enzymes. These enzymes play an important role in the metabolism of exogenous compounds, such as drugs and xenobiotics. Altered activity or expression of CYP450 enzymes may lead to adverse drug effects and toxicity. The objective of this study was to evaluate the influence of three carbon nanostructures on the activity and expression at the mRNA and protein levels of CYP2C9 isoenzyme from the CYP2C subfamily: Diamond nanoparticles, graphite nanoparticles, and graphene oxide platelets. The experiments were conducted using two in vitro models. A microsome model was used to assess the influence of the three-carbon nanostructures on the activity of the CYP2C9 isoenzyme. The CYP2C9 gene expression at the mRNA and protein levels was determined using a hepatoma-derived cell line HepG2. The experiments have shown that all examined nanostructures inhibit the enzymatic activity of the studied isoenzymes. Moreover, a decrease in the expression at the mRNA and protein levels was also observed. This indicates that despite low toxicity, the nanostructures can alter the enzymatic function of CYP450 enzymes, and the molecular pathways involved in their expression.

Highlights

  • Carbon nanostructures have unique physical, chemical, and electrical properties that make them great candidates for various biomedical applications

  • The objective of this study was to investigate the influence of carbon nanostructures, such as diamond nanoparticles (DN), graphite nanoparticles (GN), and graphene oxide platelets (GO), on enzymatic activity and expression at the mRNA and protein levels of CYP2C9

  • The highest stability was demonstrated by GO, and the lowest stability was demonstrated by GN

Read more

Summary

Introduction

Carbon nanostructures have unique physical, chemical, and electrical properties that make them great candidates for various biomedical applications. Due to their structure, they can be functionalized with different types of chemical compounds, and with biomolecules [1,2,3]. It was reported that carbon nanostructures administered intraperitoneally can be transferred and deposited in the liver. When they have accumulated, they do not cause a toxic effect on the liver and show no effect on overall animal health status [7,8]

Objectives
Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call