Influence of sample application mode on performance of pressurized planar electrochromatography in completely closed system

Abstract

Three modes of sample application on the chromatographic plate are applied at present investigations of pressurized planar electrochromatography (PPEC) systems taking into special attention their influence on performance of the separating system. These modes are as follows: application of the sample solution directly on the chromatographic plate with microsyringe, deposition of sample solution on scrap of adsorbent layer followed by location oft this scrap on the chromatographic plate, application of the sample solution with commercially available aerosol applicator. These modes were combined with prewetting procedures of the chromatographic plates which lead to an accomplishment of equilibration of the stationary phase–mobile phase system. The plots of plate height versus linear flow rate of the mobile phase are presented for PPEC systems for the first time. The best separation performance has been obtained in PPEC system when prewetting of the chromatographic plate followed the sample application with commercially available aerosol applicator. The higher repeatability of migration distance of the solute bands has been obtained in PPEC experiments when the sample application was followed by prewetting the chromatographic plate in comparison to the experiments when these operations were performed in reversed order.