Influence of salinity and alkalinity on serum ion concentrations, serum osmosis and gill Na+/K+-ATPase activity of Barbus capito

Abstract

In this paper,the serum osmosis,serum ions concentration and Na+/K-ATPase activity in branchial epithelium of Barbus capito were investigated in a ambient of salinity,alkalinity and orthogonal experiment.The results showed that osmolarity in serum and serum ions concentration(Na+,K+,Cl-)were elevated significantly with the rising salinities(8,10,12,14g/L).The gill Na+/K+-ATPase activity increased in the beginning and decreased subsequently,Na+/K+-ATPase activity was positively related to the difference between osmolarity in water and serum osmolarity.The concentration of K+ and urea nitrogen were elevated significantly with the rising alkalinity(19.05,30.20,47.86,75.86mmol/L),but serum osmolarity and Na+/K+-ATPase in branchial epithelium were not significantly different.In Two-Factor test,the serum osmolarity and concentration of serum ions(Na+,Cl-,urea nitrogen) were elevated significantly with interaction of salinity and alkalinity,Na+/K+-ATPase activity was influenced significantly by salinity,alkalinity and interaction,the influence degree of the three effects the salinity was the greatest,followed by alkalinity,interaction.The gill Na+/K+-ATPase activity increased in the beginning and decreased subsequently,the highest value of ATPase was in orthogonal group of salinity 10g/L and alkalinity 30.20mmol/L.We can draw a conclusion that Barbus capito can survive in single salinity below 12g/L and two factor of salinity 10g/L and alkalinity 30.20mmol/L.