Abstract
Herein, coverslips were used as solid supports for the synthesis of gold nanoparticles (AuNPs) in three steps: (i) detergent cleaning, (ii) evaporation of 4 nm gold film and (iii) exposure at high annealing temperature (550 °C) for 3 h. Such active gold nanostructured supports were investigated for their stability performances in aqueous saline buffers for new assessments of chemical sensing. Two model buffers, namely saline-sodium phosphate-EDTA buffer (SSPE) and phosphate buffer saline (PBS), that are often used in the construction of (bio)sensors, are selected for the optical and microscopic investigations of their influence over the stability of annealed AuNPs on coverslips when using a dropping procedure under dry and wet media working conditions. A study over five weeks monitoring the evolution of the localized surface plasmon resonance (LSPR) chemosensing of 1,2-bis-(4-pyridyl)-ethene (BPE) is discussed. It is concluded that the optimal sensing configuration is based on annealed AuNPs exposed to saline buffers under wet media conditions (overnight at 4 °C) and functionalized with BPE concentrations (10−3–10−11 M) with the highest LSPR spectra after two weeks.
Highlights
Metallic nanoparticles are attractive active supports for various optical sensing applications [1,2,3]
The present paper reports on the influence of the aqueous buffers sodium phosphate-EDTA buffer (SSPE) and phosphate buffer saline (PBS) over the stability of high-annealed 4 nm gold film-coated coverslips using on optimized AuNPs synthesis protocol [29]
Nanostructured coverslips were characterized with a scanning electron microscope (SEM) (FEG-SU8030, Tokyo, Japan) and an atomic force microscope (AFM) (Bruker ICON, Billerica, MA, USA) with cantilever ScanAsyst-Air in silicon nitride with a tip height of 2.5–8.0 mm
Summary
Metallic nanoparticles are attractive active supports for various optical (bio) sensing applications [1,2,3]. AuNPs represent excellent nanoplatforms in the development of various configurations of sensing chemicals and biomolecules including viruses [8,9] In this context, the localized surface plasmon resonance (LSPR) approach is often used in (bio)sensing diagnostics by monitoring the changes in AuNPs size, shape, composition, inter-particle distance, dielectric constant (refractive index) of the surrounding medium [8,10] and the linear and/or non-linear optical properties [11]. The present paper reports on the influence of the aqueous buffers SSPE and PBS over the stability of high-annealed 4 nm gold film-coated coverslips using on optimized AuNPs synthesis protocol [29]. An example of BPE (1,2-bis-(4-pyridyl)-ethene) chemical sensing under optimal conditions is discussed
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