Abstract
ABSTRACT Al2O3/Ce-tetragonal zirconia polycrystal (TZP) nanocomposite was synthesized by a colloidal processing route and sintered in air atmosphere. Sandblasting treatment was made to alumina toughened zirconia (ATZ) nanocomposite in order to evaluate the influence of surface roughness on the osteogenic differentiation performing in vitro growing a human osteoblast-like cell line, SaOs-2, and human adipose-derived mesenchymal stem cells (hADMSC) osteogenic differentiated. Smooth roughness values around Ra = 0.5 µm were obtained when the abrasive material was below 90 µm increasing the expression of BGLAP and IBSP genes and Ra = 1.5 µm was found with particles of sizes between 90 and 250 µm upregulating SPARC gene. The non-cytotoxicity and haemocompatibility of ATZ nanocomposite were proved. Alumina-ceria-stabilized zirconia nanocomposite presented in this work exhibits a high potential for application in the fabrication of dental implants due to their biological behavior and very promising mechanical properties.
Highlights
During more than 40 years, commercially pure titanium and titanium alloys were widely used as dental implant materials due to their excellent biocompatibility, early osseointegration and high corrosion resistance [1]
An increase in the expression of BGLAP and IBSP genes was observed on samples sandblasted with white corundum (< 90 μm) whereas SPARC gene was upregulated on samples sandblasted with SiC (90–100 μm)
According to the results obtained in the cytotoxicity and heamolysis experiments, the data obtained in our study revealed that there are no significant differences in the responses of osteogenic cells and Human Adipose-derived mesenchymal stem cells (hADMSC) towards the different surface modification treatments in terms of biocompatibility
Summary
During more than 40 years, commercially pure titanium and titanium alloys were widely used as dental implant materials due to their excellent biocompatibility, early osseointegration and high corrosion resistance [1]. Bioceramic materials offer excellent opportunities to combine the absence of metal ions, good bone ingrowth characteristics and improved aesthetics due to the possibility of dying the product with pigments In this context, alumina (Al2O3) was the first bioceramic used as an implant material [4], due to its low friction, wettability, wear resistance and biocompatibility. The purpose of the present study was to perform in vitro osteogenic differentiation assays growing a human osteoblast-like cell line, SaOs-2, and hADMSCs on different ATZ supports in order to determine the influence of the composition and roughness surface on the behavior of the cells in relation with these new implants In this context, different tests were performed to study cytotoxicity, viability, haemolysis and differences in terms of osteogenic and apoptotic gene expression between the samples
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