Abstract
Some parameters of tomato fruits ripening such as color, pH, oBrix, acidity and lycopene and β-carotene content were evaluated during the ripening of fruits. Five pesticides were quantified in organic extracts derived from tomatoes at different stages of maturation. The solid-liquid extraction technique with partition at low temperature (SLE-PLT) was used to obtain these organic extracts. The matrix effect of tomato was calculated from the results of analysis by gas chromatography with electron capture detector (GC-ECD). Correlations between maturation parameters and matrix effect were performed. The correlation between the content of carotenoids and matrix effect was high for all pesticides studied. The results indicate that the effect produced by the tomato matrix is correlated with the presence of lycopene and β-carotene in organic extracts. The principal component analysis (PCA) showed that lycopene, carotene and the mixture of both have significant effects on the chromatographic response of pesticides, separating them into distinct groups.
Highlights
The tomato (Lycopersiconesculentum Mill) is one of the vegetable with a large market in Brazil and is much appreciated for its high nutritional value
Effect of the components of tomato Standard solutions containing the five pesticides in the concentration of 100 μg L-1 in acetonitrile and in organic extracts of the matrix were analyzed by gas chromatography with electron capture detector (GC-electron capture detector (ECD))
Previous works have shown the influence of the tomato components in chromatographic responses of the pesticides during the analysis by gas chromatography (GC).[9,10]
Summary
The tomato (Lycopersiconesculentum Mill) is one of the vegetable with a large market in Brazil and is much appreciated for its high nutritional value. The quantifications of lycopene and β-carotene in matrix extracts, in several stages of maturation, were performed in spectrophotometer with wavelength of 470 nm.[2] Analytical curves were obtained at concentrations between 0.25 and 2.5 mg L-1 in acetonitrile. Effect of the components of tomato Standard solutions containing the five pesticides in the concentration of 100 μg L-1 in acetonitrile and in organic extracts of the matrix were analyzed by GC-ECD.
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