Influence of repeated prescribed burning on incorporation of 13C from cellulose by forest soil fungi as determined by RNA stable isotope probing

Abstract

Repeated prescribed burning is frequently used as a forest management tool and can influence soil microbial diversity and activity. Soil fungi play key roles in carbon and nutrient cycling processes and soil fungal community structure has been shown to alter with increasing burning frequency. Such changes are accompanied by changes to soil carbon and nitrogen pools, however, we know little regarding how repeated prescribed burning alters functional diversity in soil fungal communities. We amended soil with 13C-cellulose and used RNA stable isotope probing to investigate the effect of biennial repeated prescribed burning over a 34-year period on cellulolytic soil fungi. Results indicated that repeated burning altered fungal community structure. Moreover, fungal community structure and diversity in 12C and 13C fractions from the unburned soil were not significantly different from each other, while those from the biennial burned soils differed from each other. The data indicate that fewer active fungi in the biennially burned soil incorporated 13C from the labelled cellulose and that repeated prescribed burning had a significant impact on the diversity of an important functional group of soil fungi (cellulolytic fungi) that are key drivers of forest soil decomposition and carbon cycling processes.