Influence of redox potential on the accumulation of poly(3-hydroxybutyrate) by Bacillus megaterium.

Abstract

The main goal of the present study was to evaluate the oxidation-reduction potential (ORP) on the production of poly(3-hydroxybutyrate) (P(3HB)) by Bacillus megaterium. Each microorganism has an optimal ORP range, and changes to the culture medium's ORP may redistribute the cell's metabolic flux, as such, the measurement and control of the ORP profile allows one to, in a way, manipulate the microbial metabolism, affecting the expression of certain enzymes and allowing for better control over the fermentative process. The ORP tests were carried out in a fermentation vessel coupled with an ORP probe, containing 1L of mineral medium added with agroindustry byproducts (60% v/v of confectionery wastewater, and 40% v/v of rice parboiling water). The system's temperature was kept at 30°C, with an agitation speed of 500rpm. The vessel's airflow rate was controlled via a solenoid pump based on the ORP probe's data. Different ORP values were evaluated to verify their impact on biomass and polymer production. Cultures using OPR levels of 0mV displayed the highest amounts of total biomass (5.00gL-1) when compared to -20mV and -40mV (2.90g L-1 and 0.53g L-1, respectively). Similar results were also found for P(3HB)-to-biomass ratio, with polymer concentration being reduced when using ORP levels below 0mV and with a maximum amount of polymer-to-biomass ratio of 69.87% after 48h of culture. Furthermore, it was possible to observe that the culture's pH can also affect total biomass and polymer concentration, albeit to a lesser extent. Thus, when considering the data found during this study, it is possible to observe that ORP values can greatly impact B. megaterium cell's metabolism. Furthermore, the measurement and control of ORP levels may be an invaluable asset when trying to maximize polymer production under different culture conditions.