Influence of red alder on soil nitrogen transformations in two conifer forests of contrasting productivity

Abstract

We conducted laboratory studies to determine the effects of red alder ( Alnus rubra Bong.) on soil N transformations and N availability indices at two conifer forest sites of contrasting productivity. The inclusion of red alder in conifer forests significantly increased gross rates of N mineralization, N immobilization, nitrification and NO 3 − immobilization, and the effects of alder were generally similar for soils from low- and high-productivity sites. However, the addition of alder to the conifer stand at the high productivity site increased gross N mineralization and immobilization processes more than at the low productivity site. At both sites, gross N and NO 3 − production were enhanced by alder more than gross N immobilization processes, leading to higher rates of net N mineralization and nitrification. At the fertile site, most microbial N assimilation occurred from the NO 3 − pool, compared with less than half at the infertile site (none as NO 3 − in the less productive pure conifer stand). Heterotrophic nitrification (as indicated by a lack of C 2H 2 inhibition) accounted for 65–72% of the gross nitrification in all stands that exhibited nitrification (no nitrification was detected in the pure conifer stand at the infertile site). The inclusion of red alder had no effect on the proportion of total nitrification that was heterotrophic, despite the lower soil pH in mixed alder-conifer stands compared to conifer stands. Gross rates of N mineralization correlated well with both autotrophic and heterotrophic nitrification across all soils. Gross N mineralization may be a good index of NH 4 + availability to autotrophic nitrifiers, as well as the quality of organic N as a substrate for heterotrophic nitrification. Most estimates of microbial biomass and activity, N availability and N transformation rates were significantly correlated with each other. In general, gross N transformations were better correlated with other indices of N availability and microbial activity than estimates of net N transformations. Similar N cycling rates and microbial biomass N pool sizes in pure alder and adjacent alder-conifer stands at the fertile site suggest that continued inputs of N via symbiotic N-fixation by red alder in coniferous forest stands can lead to the elimination of N-limitation to forest ecosystem production.