Influence of Reactive Oxygen Species on the Enzyme Stability and Activity in the Presence of Ionic Liquids

Pankaj Attri,Eun Ha Choi,Andrew C Marr

doi:10.1371/journal.pone.0075096

Pankaj Attri, Eun Ha Choi + Show 1 more

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https://doi.org/10.1371/journal.pone.0075096

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Journal: PloS one	Publication Date: Sep 16, 2013
Citations: 99	License type: CC BY 4.0

Affiliation: Kwangwoon University

Abstract

In this paper, we have examined the effect of ammonium and imidazolium based ionic liquids (ILs) on the stability and activity of proteolytic enzyme α-chymotrypsin (CT) in the presence of cold atmospheric pressure plasma jet (APPJ). The present work aims to illustrate the state of art implementing the combined action of ILs and APPJ on the enzyme stability and activity. Our circular dichroism (CD), fluorescence and enzyme activity results of CT have revealed that buffer and all studied ILs {triethylammonium hydrogen sulphate (TEAS) from ammonium family and 1-butyl-3-methyl imidazolium chloride ([Bmim][Cl]), 1-methylimidazolium chloride ([Mim][Cl]) from imidazolium family} are notable to act as protective agents against the deleterious action of the APPJ, except triethylammonium dihydrogen phosphate (TEAP) ammonium IL. However, TEAP attenuates strongly the deleterious action of reactive oxygen species (ROS) created by APPJ on native structure of CT. Further, TEAP is able to retain the enzymatic activity after APPJ exposure which is absent in all the other systems.This study provides the first combined effect of APPJ and ILs on biomolecules that may generate many theoretical and experimental opportunities. Through this methodology, we can utilise both enzyme and plasma simultaneously without affecting the enzyme structure and activity on the material surface; which can prove to be applicable in various fields.

Highlights

The production and applications of enzymes have rapidly increased, in biochemical research, while it affects chemical, food and pharmaceutical industries, due to exquisite biological activities of enzymes [1,2,3]
We have explored the effect of two families of ionic liquids (ILs), such as triethylammonium dihydrogen phosphate (TEAP) and triethylammonium hydrogen sulphate (TEAS) from the ammonium family and 1-butyl-3-methyl imidazolium chloride ([Bmim][Cl]), 1-methylimidazolium chloride ([Mim][Cl]) from the imidazolium family on CT in the presence of atmospheric pressure plasma jet (APPJ)
This paper presents the first investigation revealing that ILs can protect the enzyme structure and activity against the deleterious action of reactive oxygen species (ROS) produced by APPJ

Summary

Introduction

The production and applications of enzymes have rapidly increased, in biochemical research, while it affects chemical, food and pharmaceutical industries, due to exquisite biological activities of enzymes [1,2,3]. Enzymes 3D structures have several weak interactions such as ionic effects, hydrogen bonding and hydrophobic interactions which can be disturbed by small changes in the environment of the enzymes [1,2]. Such small changes can result in denaturation or inactivation of the enzymes via unfolding [4,5,6]. Several strategies have been proposed so far in order to prevent denaturation of enzymes [7,8,9,10,11], which include chemical modification, immobilization, genetic modification and use of stabilizing agents. To our knowledge there has been no previous study regarding the protective action of ILs against reactive oxygen species (ROS) produced by atmospheric pressure plasma jet (APPJ)

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