Abstract
The chest X-ray is a simple technique for routine medical examinations. Patients under investigation (PUI) might be exposed to radiation over the recommended dose due to the necessity of repeated X-rays. The aim of this research was to study the effects of repeated chest X-rays on peripheral blood mononuclear cells (PBMCs). The study determined characteristics of PBMCs by inverted microscope and flow cytometry after single and repeated X-ray exposure doses. The morphology of PBMCs showed cell differentiation to mesenchymal stromal cells throughout the plate and the trend of cells to differentiate in control groups more than single exposure and repeated irradiation. The cell growth of PBMCs was decreased after 10 days following repeated X-ray exposure as compared to controls. The overall results indicated that low-dose radiation from diagnostic X-rays affected PBMCs possibly indicating harmful radiation exposure. Future clinical studies will necessitate a larger sample size to more clearly assess cellular changes.
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