Influence of prostaglandins on glucose transport in isolated rat uterus

Abstract

Glucose transport by uterine strips from ovariectomized estrogenized rats was explored. Sugar transport was significantly different from sacarose values (non-specific diffusion) only after 60 min of incubation. The addition of cytochalasin B demonstrated that we are measuring a specific mechanism for glucose transport. Insulin-enhanced sugar transport only at 0.5 or 0.25 U/ml prostaglandin E 1 (PGE 1), PGE 2 and PGF 2α (10 −7 M) significantly improved glucose transport, but indomethacin (10 −6 M) failed in modifying this parameter in either control nor insulin-treated tissues. We did not observe an additive or synergistic action between PGE 2 (10 −7 M) and insulin (used at maximal or submaximal concentration).