Abstract
A procedure was developed to investigate the electrolyte metabolism of human trabecular bone and its regulation in vitro, in particular the influence of prostaglandins. Trabecular bone was prepared from femoral heads of patients who had undergone hip replacement surgery for coxarthrosis. 500 mg samples were incubated in modified EAGLE's minimal essential medium. Net electrolyte movements between bone and incubation medium were measured. During 6 hours of incubation PGE 2 caused an increase in the release of calcium and magnesium from bone into incubation medium as compared to controls. The effect of PGE 2 was dose-dependent and comparable to that of human parathyroid hormone 1–34 (hPTH 1–34) whereas hPTH 3–34 had no effect. Human calcitonin (hCT) caused a decrease in the release of calcium and magnesium. PGE 2 was found to be the most potent prostaglandin. PGE 1 and PGE 2α had about 50% and PGE 1α about 40% of the potency of PGE 2. PGA 1 and PGA 2 had no effect. The effect of PGE 2 could be completely inhibited by hCT and was not further enhanced by hPTH 1–34. Magnesium movement was affected in the same way as calcium movement, while phosphate movement and release of alkaline phosphatase and hydroxyproline from bone into incubation medium were not affected by prostaglandins.
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