Influence of propofol on proliferation and apoptosis of human lung cancer A549 cells

Abstract

Objective To investigate the influence of propofol on lung cancer cell proliferation and apoptosis in vitro. Methods MTT assay was used to detect the inhibition effect of propofol on the growth of A549. Flow cytometry was applied to detect the impact of propofol on apoptosis of A549 cells. Western blotting was used to determine the effect of propofol on aquaporin 1(AQP1) protein expression in A549 cells, and the migration ability of A549 cells was investigated by Transwell chamber. Results MTT assay and flow cytometry showed that the rate of inhibition and apoptosis in the experiment group after propofol acting on A549 were(52.8±5.2)%,(61.2±5.9)%, (69.4±6.1)%;(42.8±4.1)%,(59.8±11.2)%,(71.4±13.4)%, respectively. The expression of AQP1 protein was 0.87±0.12、0.54±0.07、0.31±0.04;and the migration ability of A549 cells was 1.35±8.98、51.78±7.58、40.35±5.12. Conclusion Propofol can significantly inhibit the proliferation, apoptosis and migration ability of human lung cancer A549 cells, which may be associated with the down- regulation of AQP1 protein. Key words: Propofol; Lung cancer; Apoptosis; Aquaporin 1