Abstract
The performance of hematological tests deteriorates with the increase in the length of sample preservation. Therefore it has been an issue to characterize the maximum permissible period spent between blood collection and measurement to have the acceptable test report. From this view point, a study was undertaken to know about the effect of preservation length on complete blood count (CBC) in rat of Long Evans strain. A total of 30 samples were collected from 10 apparently healthy rats aged between 45-48 days and the blood samples were kept in commercial test tubes treated with EDTA. The test tubes containing whole blood samples were divided into three different groups based on preservation length and were allowed to keep at 4ºC for three different lengths of time viz. 2 hours, 4 hours and 6 hours until analysis. The samples were then analyzed for their complete blood count (TEC, TLC, Hb, PCV, DLC, Absolute Leukocyte Count, Red Cell Indices, RDW-SD, RDWCV, Platelet, MPV, PCT and PDW) using Sysmex XT-1800i auto hematological analyzer. Result showed that no significant change in CBC with the variation in preservation length. Based on these findings, it can be concluded that blood samples can be preserved for as long as 6 hours to have the same report obtainable when the samples are preserved at 4ºC in refrigerated condition for 2 or 4 hours.
 Asian J. Med. Biol. Res. March 2020, 6(1): 22-26
Highlights
The determination of the effects of storage on complete blood count in different anticoagulant is an aspect of quality assurance
Effects on total erythrocyte count and erythrocyte indices The values of different blood parameters (TEC, Hb, Hct, MCV, MCH and MCHC) placed in Table 1 showed that no significant differences among them at different length of preservation (2h, 4h and 6h) of the samples in refrigerated condition at 4oC
Effects on total leukocyte count (TLC) and absolute count of its cell component Table 2 showed that the absolute value of WBC and its component cell viz. neutrophil, lymphocyte, monocyte, eosinophil and basophil
Summary
The determination of the effects of storage on complete blood count in different anticoagulant is an aspect of quality assurance. Quality assurance involves the application of all possible means to guarantee that the results reported by the laboratory are both reliable and valid. Excessive delay in processing blood samples for hematological testing could compromise the reliability of the result. It is well known that prolonged storage could compromise red blood cell (RBC) properties, in particular storage condition could lead to metabolic depletion, disturbed ion homeostasis, protein and lipid modifications (e.g., oxidation, degradation, cross-linking) accompanied by alterations in intracellular hemoglobin concentrations (Ho et al, 2003). It is routinely recommended that hematological determinations on blood samples are carried out immediately after blood collection, and if not possible, the samples should be refrigerated until determination to minimize artifactual changes (Wood et al, 1999).The components necessary for blood storage are the anticoagulant and refrigerator.
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