Abstract
In spite of intensively conducted research allowing for the development of more and more advanced wound dressing materials, there is still a need for dressings that stimulate not only reparative and regenerative processes, but also have a positive effect on infected and/or difficult-to-heal wounds. Porous dressing materials based on butyric-acetic chitin co-polyester containing 90% of butyryl and 10% of acetyl groups (BAC 90/10) can also be included in the group mentioned above. Two types of dressings were obtained by the salt leaching method, i.e. a porous sponge Medisorb R and Medisorb Ag with an antibacterial additive. The aim of the study was to evaluate biological effects of porous Medisorb R and Medisorb Ag dressings under in vitro and in vivo conditions. In an in vitro biodegradation test, no mass loss of Medisorb R dressing was observed within 14 days of incubation in physiological fluids at 37 °C. However, on the basis of the FTIR (Fourier Transform Infrared Spectroscopy) tests, surface degradation of Medisorb R dressing was observed. Additionally, the antibacterial activity of the porous Medisorb Ag dressing containing microsilver as an antibacterial additive was confirmed. The in vivo studies included inflammatory activity, skin irritation and sensitisation tests, as well an assessment of local effect after contact with subcutaneous tissue up to 6 months and skin wounds up to 21 days. In the in vivo tests, the dressings exhibited neither effects of skin irritation nor sensitisation. Under macroscopic examination, in full thickness defects of subcutaneous tissue and skin, the dressings caused wound healing with no inflammation, undergoing the most gradual biodegradation between weeks 4 and 8, and the observed differences were statistically significant. In the histological assessment, a weakened, limited inflammatory process associated with degradation of the material has been observed. The process of skin wound healing under Medisorb R dressing in the early period was accelerated compared to that observed in the control group with a gauze dressing.
Highlights
Wound healing is a spontaneous process that can be hampered in the case of large, long-lasting and difficult-to-treat chronic wounds
The conducted research proves that porous dressing materials Medisorb R and Medisorb Ag are safe materials used in wound healing process
Medisorb R undergoes slow degradation in human plasma, which was confirmed using FTIR tests, in tests based on material mass determination in physiological fluids, no mass loss was observed
Summary
Wound healing is a spontaneous process that can be hampered in the case of large, long-lasting and difficult-to-treat chronic wounds. In such cases, if the autologous skin transplants are not available, biopolymers are used, which show the ability to initiate and stimulate the appropriate process of skin and epidermis healing [1]. Among many factors which influence the wound healing process, an extracellular matrix (ECM) plays a key role in the organization and management of cell phenotype, adhesion, migration and proliferation. The ECM contains molecules synthesized by fibroblasts including proteoglycans such as chondroitin sulphate, keratin sulphate, heparin sulphate, and fibrous proteins such as laminin, collagen and elastin. The biopolymers presented below meet the biocompatibility requirements [13,14]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
