Abstract
For back disorders, cell therapy is one approach for a real regeneration of a degenerated nucleus pulposus. Human mesenchymal stem cells (hMSC) could be differentiated into nucleus pulposus (NP)-like cells and used for cell therapy. Therefore it is necessary to find a suitable biocompatible matrix, which supports differentiation. It could be shown that a differentiation of hMSC in a microbial transglutaminase cross-linked gelatin matrix is possible, but resulted in a more chondrocyte-like cell type. The addition of porcine NP extract to the gelatin matrix caused a differentiation closer to the desired NP cell phenotype. This concludes that a hydrogel containing NP extract without any other supplements could be suitable for differentiation of hMSCs into NP cells. The NP extract itself can be cross-linked by transglutaminase to build a hydrogel free of NP atypical substrates. As shown by side-specific biotinylation, the NP extract contains molecules with free glutamine and lysine residues available for the transglutaminase.
Highlights
Back disorders affect many people in industrialized countries and are a large economic problem
The current study investigates the influence of different matrices on mesenchymal stem cell differentiation
The first aim of the study was to investigate whether a differentiation of Human mesenchymal stem cells (hMSC)-TERT in TGase cross-lined gelatin is possible and if the cells develop an nucleus pulposus (NP)-like cell phenotype when stimulated with the growth factor TGF- 3 according to the standard differentiation protocol
Summary
Back disorders affect many people in industrialized countries and are a large economic problem. These medical conditions can be caused by the degeneration of the intervertebral disc (IVD), the nucleus pulposus (NP). In contrast to NP cells, these pluripotent stem cells have been found in almost every organ in adulthood These cells are of high plasticity and have the capacity of multilineage differentiation. They are accessible in sufficient quantities from bone marrow and fat tissue and comparably easy to expand and manipulate which make them ideal candidates for cell-based IVD regeneration [1]
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