Influence of polymorphisms in chemokine receptor-5(CCR5) and CCR2 on susceptibility to HIV, viral load and CD4 count with anti-retroviral therapy

Abstract

Event Abstract Back to Event Influence of polymorphisms in chemokine receptor-5(CCR5) and CCR2 on susceptibility to HIV, viral load and CD4 count with anti-retroviral therapy Irma Izani Mohamad Isa1 and Suhaili Abu Bakar1* 1 Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia Background Genetic variation of the host can influence susceptibility and pathogenesis of some diseases including HIV/AIDS. CCR5 is a HIV co-receptor used by the virus for entry to the host cells. Certain polymorphisms in CCR5 and CCR2 can provide protection against HIV infection and lead to slower rate of progression to AIDS. This study aimed to investigate the CCR5-Δ32, CCR5-R223Q and CCR2-V64I mutations with susceptibility to HIV, viral load suppression and CD4 recovery following highly active anti-retroviral therapy (HAART) in Malaysian population. Methods Ethical approval was obtained from the ethics committees of medical research (MREC) and UPM. 175 HIV-infected and 150 healthy subjects of Malay, Chinese and Indian ethnics were recruited from out-patient clinics of three selected hospitals and university areas respectively. CCR5-Δ32 was genotyped by polymerase chain reaction (PCR) while CCR5-R223Q and CCR2-V64I were identified using PCR-restriction fragment length polymorphism (RFLP). CD4 count and viral load were extracted from medical record and followed for up to 18-24 months after the initiation of HAART. T-test/one-way ANOVA and chi-squared test were used in CD4 and viral load analyses respectively. Results CCR5-Δ32, CCR5-R223Q and CCR2-V62I were not significantly different in healthy as compared to HIV-infected subjects, indicating the absence of protective effect of these polymorphism to HIV infection. Both CD4 recovery and viral load suppression were also not associated with CCR5-Δ32, CCR5-R223Q and CCR2-V62I. Conclusion CCR5-Δ32, CCR5-R223Q and CCR2-V62I have no impact on HIV susceptibility, CD4 recovery and viral load in Malaysian population. Further studies are needed to identify other host genetic variants that can explain variation in individuals’ response to HAART towards precision medicine for the treatment of HIV infection Keywords: CCR5, CCR2, HIV, CD4, Viral Load Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Infectious diseases Citation: Mohamad Isa I and Abu Bakar S (2019). Influence of polymorphisms in chemokine receptor-5(CCR5) and CCR2 on susceptibility to HIV, viral load and CD4 count with anti-retroviral therapy. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00139 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Suhaili Abu Bakar, Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Malaysia, suhaili_ab@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Irma Izani Mohamad Isa Suhaili Abu Bakar Google Irma Izani Mohamad Isa Suhaili Abu Bakar Google Scholar Irma Izani Mohamad Isa Suhaili Abu Bakar PubMed Irma Izani Mohamad Isa Suhaili Abu Bakar Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.