Influence of polymorphic variants of the leptin gene receptor on diabetes mellitus 2 type and obesity

Abstract

The aim of this work is to study the role of LEPR gene polymorphism rs1137101 on the development of diabetes mellitus type 2 and its influence in developing obesity in these patients. Materials and methods. 103 patients with type 2 diabetes clinical complications were evaluated. The control group comprised of 100 people of corresponding age without diabetes and obesity. The analysis of DNA loci polymorphism was carried out by the method of polymerase chain reaction in real time (TaqManR SNP Genotyping Assay). Blood leptin was determined (DRG International Inc., USA).Results and discussion. Results showed that allelic polymorphism rs1137101 (Gln223Arg) of LEPR gene had been associated with the development of diabetes mellitus type 2: allele 223Gln promotes 1.5 times, and allele 223Arg – reduces 1.5 times (p=0,039) the risk for developing type 2 diabetes. The presence of allele223Gln in patients with type 2 diabetes assists in developing obesity, specifically – higher values of body mass index (26,29Ѓ}0,81 kg/m2 in comparison to 30,32Ѓ}0,68 kg/m2; F=7,51; p=0,003) and leptin blood level (87,27Ѓ}7,52 ng/ml in comparison to 105,58Ѓ}10,25 ng/ml; F=6,59; p=0,006). Allele 223Gln is associated with obesity in diabetics with BMI >30 kg/ m2. In carriers of allele 223Gln the risk of developing obesity is 1,8 times higher (OR=1,811; CI=1,036-3,165; р(χ2)=0,038).Conclusions. Polymorphism rs1137101 of LEPR gene is a genetic risk factor for diabetes mellitus type 2 and promotes development of obesity in these patients