Abstract

Somatic embryogenesis has been reported in different papaya cultivars across the world. However, all report suggests indirect embryogenesis in papaya with minimum of 3-6 months required for embryo formation. We have investigated role of polyamines (spermidine, spermine and putriscine) on direct embryogenesis on immature zygotic embryos excised from papaya cultivar Pusa Delicious. A brief exposure (2-4 weeks) of putriscine on induction medium (100 μM) augmented direct embryogenesis on explant. However, prolonged exposure of putriscine was found detrimental.

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