Abstract

The present work is focused on investigating the effect of plasma-activated water (PAW) on the seed germination and seedling growth of radish, fenugreek, and pea seeds. A home-made gliding arc discharge (GAD) reactor generated in air under atmospheric pressure and operating at 50 Hz line frequency is used for water activation. PAW was prepared by exposing deionized water to GAD for 5, 10, 15, and 20 min. As a result of plasma treatment, deionized water showed substantial changes in physical characteristics such as pH and electrical conductivity along with chemical parameters such as nitrites, nitrates, and ammonia. The effect on germination was evaluated based on the fraction of germinated seeds and their length at 7 and 12 days after treatment. Seeds irrigated with PAW resulted in a higher imbibition rate than those irrigated by deionized water. When the seeds were irrigated with PAW, the germination parameters such as final germination rate, mean germination rate, coefficient of velocity of germination, germination index, and growth parameters such as shoot/seedling length and vigor index increased. In conclusion, PAW may be utilized to increase the germination rate of seeds.

Highlights

  • The study of the effects of low temperature plasmas on biological systems has flourished in the last two decades

  • A home-made gliding arc discharge (GAD) reactor generated in air under atmospheric pressure and operating at 50 Hz line frequency is used for water activation

  • We investigated the potential use of plasma-activated water (PAW), prepared using a cost-effective home-made gliding arc discharge (GAD) generated at 50 Hz line frequency using air as a carrier gas, and its proliferation effect on seed germination and growth of radish, fenugreek, and pea

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Summary

INTRODUCTION

The study of the effects of low temperature plasmas on biological systems has flourished in the last two decades. In the sector of agriculture, research has mostly concentrated on improving seed germination and plant growth, as well as clearing infections in seeds using plasma.. Studies on the impact of plasmaactivated water (PAW) on seedling germination and yield have been undertaken on different most valuable crop species throughout the world in recent years.. Even though intensive conventional agriculture helps in producing more than tripled crop yields in the last century, there has been a severe negative impact of pesticides and chemical fertilizers on the environment, which directly impacts biological diversity and environmental health by increasing pollution levels in different components such as water, soil, and air.. We investigated the potential use of PAW, prepared using a cost-effective home-made gliding arc discharge (GAD) generated at 50 Hz line frequency using air as a carrier gas, and its proliferation effect on seed germination and growth of radish, fenugreek, and pea

Experimental
Analysis of germination related characteristics
Estimation of water absorption and growth characteristics
Statistical analysis
Electrical characterization of the discharge
Physical and chemical parameters
Germination results
Influence of PAW treatment on seedling development
Estimation of vigor index I
CONCLUSIONS
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