Abstract

Dendrobium officinale Kimura et Migo has been known for a long time as a precious orchid, which is used for medicine and functional foods that are widely commercialized in the world. The nodal explants could be obtained in high efficiency, good quality and uniform seedlings on multiplication vegetative in vitro. They can be propagated in large quantities in a short time. The studied results showed that the nodal explants grew on MS medium + 10 % coconut milk + 25 g sucrose + 0.5 mg/L BA + 6 g agar/ liter create high buds. This buds were used to A B 1 cm 1,5 cm create bud clusters with the aim of improving in vitro vegetative. The best bud culture medium for the formation of clusters was MS + BA 2.0 mg/L + NAA 0.4 mg/L; MS + kinetin 1.5 mg/L + NAA 0.3 mg/L; MS + TDZ 2.0 mg/L + NAA 0.4 mg/L; MS + adenine 1.5 mg/L + NAA 0.3 mg/L. However, the addition of kinetin or TDZ to the culture medium needed a steps to extend the bud subculture after 45 days in culture. The best medium for the rooting is MS + NAA 0.5 mg/L.

Highlights

  • Dendrobium officinale Kimura et Migo has been known for a long time as a precious orchid

  • which is used for medicine

  • functional foods that are widely commercialized in the world

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Summary

TÓM TẮT

Lan Dendrobium officinale Kimura et Migo (Thạch hộc thiết bì) được biết đến từ rất lâu là giống lan quý, sử dụng làm thuốc và thực phẩm chức năng đang được thương mại hóa rộng rãi trên thế giới. Việc nhân giống bằng đoạn thân mang mầm cho hiệu quả nhân giống cao, phẩm chất cây giống tốt và đồng đều. Sự hình thành cụm chồi tốt nhất trên môi trường nuôi cấy MS + BA 2,0 mg/L + NAA (Naphthalene acetic acid) 0,4 mg/L; MS + kinetin 1,5 mg/L + NAA 0,3 mg/L; MS + TDZ (Thidiazuron) 2,0 mg/L + NAA 0,4 mg/L; MS + adenin 1,5 mg/L + NAA 0,3 mg/L. Từ khóa: Dendrobium officinale Kimura et Migo, Thạch hộc Thiết bì, nhân giống, cụm chồi

MỞ ĐẦU
Khử trùng mẫu vật
Nuôi cấy in vitro chồi cây lan Thạch hộc tía
Xử lý số liệu
Số chồi trung bình
Nghiệm thức MS
KẾT LUẬN

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