Abstract

The current research aimed to study the efficacy of plant extracts and essential oils from Rosmarinus officinalis, Thymus vulgaris, Cinnamomum zeylanicum and Syzigium aromaticum on mycelial growth and conidial germination of Fusarium oxysporum f. sp. cubense in vitro, as well as evaluation in vivo. Essential oils and extracts were applied in the culture medium (PDA) at melting point to estimate the direct effect on mycelial growth. Also, the estimation of volatile substances against mycelial growth was done. The obtained results indicated that plant extracts and essential oils of Cinnamomum zeylanicum and Syzigium aromaticum were effective at 500 ppm with direct effect against mycelial growth. The volatile plant extracts had no effect on mycelial growth, however, the volatile essential oils of Cinnamomum zeylanicum and Syzigium aromaticum showed good effects in controlling the disease in both 1000 and 2000 ppm. Meanwhile, extracts and essential oils of Cinnamomum zeylanicum and Syzigium aromaticum showed efficacy on the conidial germination. In vivo, both essential oils and extracts from Cinnamomum zeylanicum and Syzigium aromaticum, in addition to fungicide (Trifloxystrobin + Tebuconazole) which was effective in avoiding the spread of disease.

Highlights

  • The current research aimed to study the efficacy of plant extracts and essential oils from Rosmarinus officinalis, Thymus vulgaris, Cinnamomum zeylanicum and Syzigium aromaticum on mycelial growth and conidial germination of Fusarium oxysporum f. sp. cubense in vitro, as well as evaluation in vivo

  • The obtained results indicated that plant extracts and essential oils of Cinnamomum zeylanicum and Syzigium aromaticum were effective at 500 ppm with direct effect against mycelial growth

  • 3.1 Mycelial Growth under Influence of Essential Oils Incorporated into the Culture Medium

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Summary

Introduction

The current research aimed to study the efficacy of plant extracts and essential oils from Rosmarinus officinalis, Thymus vulgaris, Cinnamomum zeylanicum and Syzigium aromaticum on mycelial growth and conidial germination of Fusarium oxysporum f. sp. cubense in vitro, as well as evaluation in vivo. The current research aimed to study the efficacy of plant extracts and essential oils from Rosmarinus officinalis, Thymus vulgaris, Cinnamomum zeylanicum and Syzigium aromaticum on mycelial growth and conidial germination of Fusarium oxysporum f. The obtained results indicated that plant extracts and essential oils of Cinnamomum zeylanicum and Syzigium aromaticum were effective at 500 ppm with direct effect against mycelial growth. The volatile plant extracts had no effect on mycelial growth, the volatile essential oils of Cinnamomum zeylanicum and Syzigium aromaticum showed good effects in controlling the disease in both 1000 and 2000 ppm. According to these authors the banana wilt disease was described in Brazil at 1930 on Piracicaba (São Paulo state) and today can be found in all banana-producing regions. The external symptoms begin with yellowing of older leaves, followed by wilt of leaves and deformations starting on the petiole base

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