Influence of Pine Bark Tannin on Bacterial Pathogens Growth and Nitrogen Compounds on Changes in Composted Poultry Litter

C Arzola-Alavarez,Y Castillo-Castillo,Rc Anderson,Rc Beier,Br Min,A Arzola-Rubio,J Salinas-Chavira,Me Hume,O Ruiz-Barrera

doi:10.1590/1806-9061-2018-0911

Abstract

ABSTRACT To study the antimicrobial and uric acid-preserving activity of pine bark tannin on poultry litter composting, antibiotic-free wood chip-based poultry litter was distributed (11 g) to 50-mL conical centrifuge tubes and immediately amended with 1.3 mL0.4 M sodium phosphate buffer (control) or with 1.3 mL condensed tannins from pine bark (Pinus palustris; 9 % tannin wt/vol in water). All tubes (n = 3 tubes/treatment) were inoculated with a novobiocin and naladixic acid-resistant Salmo-nella typhimurium (STNN) to achieve 3.0 log10 CFU/g and incubated at 37oC for 3 days to simulate an initial compost period. Wildtype E. coli and the challenge STNN strain as well as concentrations of ammonia, uric acid and urea were measured on days 0 and 3. Pine bark tannin treatment decreased (p 0.05). Ammonia accumulation decreased (p 0.05). Results suggest that pine bark tannin treatment may preserve uric acid and reduce ammonia volatilization in composted litter while aiding Salmonella control.

Highlights

Poultry litter can contain dangerous and unwanted pathogenic bacteria which could be resistant to antimicrobials and requires treatment before being fed
The present study was conducted to examine the antimicrobial activity, uric acid-preserving and ammonia production of poultry litter compost treated with pine bark tannin
Uric acid was higher and ammonia was lower to achieve 3.0 log10 colony forming units (CFU)/g. (p

Summary

INTRODUCTION

Poultry litter can contain dangerous and unwanted pathogenic bacteria which could be resistant to antimicrobials and requires treatment before being fed. Additions of biochar (BC) to poultry litter composted for fertilizer lowered N losses by 52% (Steiner et al, 2009), but it did not clear that the N fixed with BC can be used effectively by ruminants. Dawson et al (1999) reported that feeding quebracho tannin influenced ruminal and post‐ruminal metabolism resulting in reduced nutrient utilization and impaired animal performance. There are no conclusive results on the mechanism that underlies the N transformation in composting poultry litter treated with pine bark tannins. The present study was conducted to examine the antimicrobial activity, uric acid-preserving and ammonia production of poultry litter compost treated with pine bark tannin

Analytical methods

RESULTS

DISCUSSION

Findings

CONCLUSIONS