Influence of Phosphorus and Cell Geometry on the Fractionation of Sulfur Isotopes by Several Species of Desulfovibrio during Microbial Sulfate Reduction.

Shikma Zaarur,Tanja Bosak,Shuhei Ono,David T Wang

doi:10.3389/fmicb.2017.00890

Shikma Zaarur, Tanja Bosak + Show 2 more

Open Access

https://doi.org/10.3389/fmicb.2017.00890

Copy DOI

Abstract

We investigated the influence of organic substrates and phosphate concentration on the rates of dissimilatory microbial sulfate reduction and the 34S/32S isotopic fractionation produced by several Desulfovibrio species. Our experiments corroborate the previously reported species-specific correlation between sulfur isotope fractionation and cell-specific sulfate reduction rates. We also identify cell size as a key factor that contributes to the species-effect of this correlation. Phosphate limitation results in larger cells and contributes to a small decrease in sulfur isotope fractionation concomitant with an apparent increase in cell-specific sulfate reduction rates. Sulfur isotope fractionation in phosphate-limited cultures asymptotically approaches a lower limit of approximately 5‰ as cell-specific sulfate reduction rates increase to >100 fmol cell−1 day−1. These experimental results test models that link the reversibilities of enzymatic steps in dissimilatory sulfate reduction to sulfur isotope fractionation and show that these models can provide consistent predictions across large variations in physiological states experienced by sulfate reducing bacteria.

Highlights

The oxidation of organic matter by sulfate reducing bacteria (SRB) is a globally distributed anaerobic process that influences the redox state of the Earth’s surface and the preservation of organic matter in sediments (Jørgensen, 1982; Westrich and Berner, 1984; Widdel and Hansen, 1992; Shen and Buick, 2004)
Cultures of D. fructosovorans grew in media with fructose, pyruvate, or lactate as the organic substrate
Cultures of D. inopinatus grown on malate produced more sulfide than the 14 mM concentration predicted by incomplete malate oxidation (Table 1), perhaps indicating that some of the malate may have been oxidized completely to CO2 or to small organic compounds

Summary

Introduction

The oxidation of organic matter by sulfate reducing bacteria (SRB) is a globally distributed anaerobic process that influences the redox state of the Earth’s surface and the preservation of organic matter in sediments (Jørgensen, 1982; Westrich and Berner, 1984; Widdel and Hansen, 1992; Shen and Buick, 2004). Microbial sulfate reduction (MSR) fractionates sulfur isotopes, producing sulfide depleted in heavier isotopes. The magnitude of this fractionation (34ε, defined in Equation 6) is a critical parameter for reconstructions of the carbon and sulfur cycles through Earth history (Holland, 1973; Garrels and Lerman, 1981; Berner, 2001; Luo et al, 2016). Dozens of studies have reported fractionations produced in pure cultures of sulfate reducing organisms under controlled laboratory conditions (e.g., Harrison and Thode, 1958; Thode et al, 1961; Kaplan and Rittenberg, 1964; Chambers et al, 1975; Chambers and Trudinger, 1979), in experiments with natural microbial populations (e.g., Canfield, 2001; Canfield et al, 2010) and under in situ conditions (e.g., Jorgensen, 1979; Rudnicki et al, 2001; Wortmann et al, 2001).

Methods

Results

Discussion

Conclusion