Abstract
The present work focus on the evaluation of crayfish protein concentrates, surplus from crayfish processing industry, in the formation and stabilization of high-oleic O/W emulsions as a function of pH values (3.0, 5.0 and 8.0) and Xanthan Gum (XG) concentrations (0.06, 0.12, 0.25 and 0.50 wt.%). The emulsion microstructure and stability were determined through droplet size distributions (DSD) and rheological measurements, which were determined by means of Small Amplitude Oscillatory Shear (SAOS) measurements, over the ageing time. In addition, backscattering measurements were carried out to describe the destabilization mechanism. DSD profiles confirm that emulsions at pH 3.0 are not flocculated and they are stable over the ageing time studied. However, at pH 5.0 and 8.0 flocculation and further coalescence phenomena takes place. Rheological characterisation reveals a strong dependence of linear viscoelastic properties on XG concentration. In addition, viscoelastic properties also suggest that electrostatic interactions leads to the formation of an enhanced protein/polysaccharide network at pH 3.0.
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